Sepragen's dedicated process chromatographic engineers and scientists have responded to this scalability problem by designing the WEDGE® radial column. The WEDGE® fully exploits the separation capability and high capacity of low cost soft media as well as the faster flow media currently available. It does this without compromising performance in terms of chromatographic separation, efficiency and productivity. WEDGE® columns incorporate the patented design of radial flow and traditional axial flow column chromatography. This permits simple linear scale-up of your bioprocess from lab scale axial columns to large-scale radial flow chromatographic columns.
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Sepragen's dedicated process chromatographic engineers and scientists have responded to this scalability problem by designing the WEDGE HIRES® radial column. The WEDGE HIRES® fully exploits the separation capability and high capacity of low cost soft media as well as the faster flow media currently available. It does this without compromising performance in terms of chromatographic separation, efficiency and productivity. WEDGE HIRES® are 15cm bed depth columns which utilize the Wedge Columns radial flow design and traditional axial flow column chromatography. This permits simple linear scale-up of your bioprocess from lab scale axial columns to large-scale radial flow chromatographic columns.
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Superflo Lab columns are based on radial flow chromatography. The columns have three annular channels created by four concentric rings. The outer most ring is the column body followed by the outer frit which is followed by the Inner frit. The inner most circle is the collector rod. The outer and inner channels have equal cross sectional areas and conduct the fluid flow from the outer channel to then inner channel and out of the column. The middle channel contains the chromatographic packing. Because of the large surface are of the outer channel and the low bed depth, Superflo columns allow for extremely high flow rates and very little pressure drop. The sample is introduced at the sample inlet where it is distributed evenly throughout the outer channel. From this outer channel, the sample flows through a porous tube (the outer frit) and into the packing material where the sample constituents are selectively bound. After an elution procedure, the sample components pass sequentially through the inner porous tube (Inner frit) of the column and out of the column outlet. This unique radial flow design makes the column suitable for ion exchange, affinity, hydrophobic, reverse phase and other forms of adsorption-desorption separations
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